Program

University of Bonn - Venusberg Campus / University Hospital Bonn
Biomedical Center (Building 13) - Lecture Hall
Venusberg-Campus 1
53127 Bonn
Germany

Thursday, September 5th, 2019

8:00 - 9:00 Registration, poster mounting, welcome coffee
9:00 - 9:10 Welcome address by Prof. Nicolas Wernert, Dean of the Medical Faculty, University of Bonn 
9:10 - 9:20 Opening remarks from the organizers
9:20 - 10:00 Serge Muyldermans: Nanobodies, a versatile research tool in medicine and biotechnology
Talk session 1: Nanobodies as diagnostic and therapeutic tools in the clinic
10:15 - 10:45 Tony Lahoutte: Radionuclide imaging and therapy with nanobodies
10:45 - 11:00 Sabrina Oliveira: Exploring the potential for immune modulation triggered by nanobody-targeted photodynamic therapy
11:00 - 11:30 Coffee Break
11:30 - 12:00 Paul van Bergen en Henegouwen: The development of VHH-drug conjugates
12:00 - 12:30 Pieterjan Debie: Design and preclinical evaluation of a single-label bimodal nanobody tracer for image-guided surgery
12:15 - 12:30 Florencia Linero: Targeting Receptor Mediated Transcytosis at the Blood-cerebrospinal Fluid Barrier: Trojan Horse VHHs for CNS Therapeutics?
12:30 - 13:00 Ursula Dietrich: Broadly neutralizing nanobodies against HIV-1 as basis for a prophylactic vaccine?
13:00 - 14:00 Lunch and poster viewing
14:00 - 15:00 Workshop session 1
1) Nanobody labeling (Hidde L. Ploegh)
2) Nanobody-based therapies (Paul van Bergen En Henegouwen)
3) Nanobody tools (Friedrich Koch-Nolte, Jan Steyaert)
4) Nanobody screening (Eric Geertsma, Pierre Lafaye)
Talk session 2: Technology development for the generation and application of nanobodies
15:15 - 15:45 Markus Seeger: Sybodies and NestLink - an integrated technology platform to target membrane proteins in the cellular context
15:45 - 16:15 Friedrich Koch-Nolte: Affinity matured nanobodies from llama IgH-transgenic mice
16:15 - 16:45 Coffee break
16:45 - 17:15 Pierre Lafaye: High throughput selection of nanobodies by droplet based microfluidic
17:15 - 17:30 Helen Farrants: Switching nanobodies on and off with small molecules
17:30 - 18:00 Hidde L. Ploegh: Nanobodies as imaging tools
18:00 - 18:15 Franck Perez: Full in vitro selection of synthetic humanized nanobodies for basic and cancer research
18:15 - 18:45 Markus Affolter: Applications of nanobodies and other protein binders in developmental biology
18:45 - 19:30 Poster pitches
19:30 - 23:00 Poster session, snacks and drinks, get-together

Friday, September 6th, 2019

8:00 - 8:30 Coffee
Talk session 3: Nanobodies in structural biology
8:30 - 8:45 Christian Loew: Engineered Nanobodies with a Lanthanide Binding Motif for Crystallographic Phasing
8:45 - 9:00 Felipe Opazo: A Rationally Designed and Highly Versatile Epitope Tag for Nanobody-based Purification, Detection and Manipulation of Proteins
9:00 - 9:30 Jan Steyaert: From Nanobodies to Megabodies for applications in cryo-EM
9:30 - 10:00 Eric Geertsma: Synthetic single domain antibodies for the conformational trapping of solute carriers
Talk session 4: Nanobodies for imaging applications
10:00 - 10:30 Helge Ewers: Nanobodies as labelling agents for superresolution microscopy
10:30 - 10:45 Ruiyao Cai: Whole-Body Nanobody Labeling, Tissue Clearing and Imaging using vDISCO
10:45 - 11:00 Dagmar Wachten: Subcellular Targeting of Optogenetic Tools using Nanobodies - High- jacking the Ciliary Targeting Machinery
11:00 - 11:30 Coffee break
11:30 - 12:00 Ulrich Rothbauer: Nano- and Chromobodies: how to connect biochemistry and cell biology
12:00 - 12:30 Heinrich Leonhardt: Tunable light and drug induced depletion of target proteins with nanobodies
12:30 - 13:00 Cristina Cardoso: The art of delivery: labeling and manipulation of intracellular targets
13:00 - 14:00 Lunch and poster viewing
14:00 - 15:00 Workshop session 2
5) Nanobody libraries (Serge Muyldermans, Markus Seeger)
6) Nanobody-based imaging (Helge Ewers, Heinrich Leonhardt)
7) Nanobody delivery (Cristina Cardoso, Ursula Dietrich)
Talk session 5: Nanobodies as tools to understand cell biology
15:15 - 15:45 Jan Gettemans: A Nanobody‐Based Approach to Amyloid Diseases: the Gelsolin Case Study
15:45 - 16:00 Coffee break
16:00 - 16:15 Mark Soave: Molecular pharmacology of a conformational-specific extracellular nanobody against CXCR4
16:15 - 16:30 Raimond Heukers: Nanobodies targeting and modulating G protein-coupled receptors outside in and inside out
16:30 - 16:45 Jan Habermann: Shark-derived nanobodies for biomedical applications
16:45 - 17:15 Florian I. Schmidt: Interrogating infection and immunity with camelid nanobodies
17:15 - 17:30 Björn Niebel: Nanobody-driven Sustained Increase Of Plasma Factor VII (a) Levels
17:30 - 17:40 Closing remarks and award of poster prizes
17:40 End of symposium

 

Tweet about us using: #BNNB2019

 

This symposium is kindly funded by the Fritz Thyssen Foundation.

 

We are grateful to our sponsors 'preclinics' and 'Jackson Immunoresearch'.